It completed a miserable evening for the five-times grand slam champion, now ranked 211, who earlier found out that she had been refused a wildcard for the French Open.
For her part Sharapova said she remains fully committed to making a successful comeback from her doping ban, regardless of what assistance she receives.
The five-time Grand Slam champion, 30, was banned for two years for using meldonium, with the penalty later reduced by the Court of Arbitration for Sport (CAS), which ruled she was not an intentional doper.
Sharapova will now have to wait until 20 June to discover whether she is among the wildcards at the All England Club. But she now has no possibility of competing in the first Grand Slam tournament to be contested since her return.
She did not comment about Giudicelli's ruling on Wednesday.
"You can get a wild card when you return from injury but you cannot get a wild card when returning from a doping suspension", Giudicelli said.
"It is now up to you, day by day, one tournament after the next, to find the strength within you to win more major titles, without owing anything to anyone".
Two-time French Open victor Sharapova was denied a wildcard, which are handed out at the discretion of tournament organisers, at Roland Garros.
While Sharapova was making her exit from Rome, the French Tennis Federation was broadcasting its decision on wild cards at Roland Garros, which begins on 28 May.
"She has complied with the sanction".
"Maria won twice here, but we have a huge (moral) responsibility", Giudicelli added.
Nadal suggested that the decision was made well ahead of its announcement and would have been taken as a strategy move by the 35-year-old's coaching team.
Sharapova secured a place in Wimbledon qualifying earlier this week with victory over Christina McHale in the first round of the Italian Open.
So he decided not to award her a wild card for the French Open. She skipped a news conference after pulling out of a second-round match in Rome on Tuesday because of a left thigh injury.
"I will be getting all the necessary examinations to make sure it's not serious".
And assuming she is fit, she is likely to want to play at least two warm-up events.
Cash said he was disappointed the Italian federation had offered up a wildcard this week but hoped there'd be no such offer from Wimbledon officials.
"This issue with Maria and the French Open, that is a effect of Wada breaching their own rules for a delinquent way of operating". What also disappoints me is the fact that WADA has not conducted their business properly.
But not everyone in the tennis community was in agreement. It's going to be a very exciting fortnight at Roland Garros. "If he does that, it's a huge travesty and a mistake".
"I wanted to do it for myself, but also for all these people", she added.